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4. Arrange chops in top chamber of steamer, and cover.
b HRP was added to the top chamber, and HRP passage was monitored over time.
b HRP was also added to the top chamber, and HRP passage was measured 2 h after HBP stimulation.
b HRP was also added to the top chamber, and HRP passage was measured 2 h after stimulation.
Briefly, HEK 293 cells were suspended in DMEM and plated in the top chamber.
For each treatment group 5×105 cells were seeded into the top chamber.
In each group, an expected 5×105 living cells were seeded into the top chamber of a Matrigel transwell.
Briefly, serum-starved MEF cells were seeded into the top chamber at a density of 1×104 cells per well.
20 000 MSC and 2 500 epithelial cells were seeded on the bottom chamber or on the top chamber, respectively.
Epithelial cell monolayers were incubated with bacteria (5×107 CFU/TW) upon the apical surface (top chamber).
Cells (5 × 10) were seeded in the top chamber.
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