Exact(18)
While they show a liquidity and the quality of growth that many investors prefer, Malloy points out that many top Bio companies are trading at multiples of two to three times the company's projected growth rate.
The bands of amplicons were then imaged under UV light using the Flo Gel FGIS-3 fluorescent gel image system (Top BIO Co., Taipei, Taiwan), and the sizes of bands were estimated using Quantity One software version 4.62 (Bio-Rad Laboratories, HerCaliforniaifornia, USA).
The Ingenuity software makes use of its own two databases, Top Bio Function and Top Canonical Pathways, which however are comparable with the GO and the KEGG databases, respectively, used by the other enrichment tools.
Among the 300 macrophage genes undergoing expression variation upon LPS stimulation, the pathway analysis knowledge database has revealed that 178 gene products could be defined as "Top Bio Functions" thanks to their score (data not shown).
The top bio functions in IPA are shown in Figure 6.
The IPA Top Canonical Pathways and Top Bio Functions analyses (Table 5) were in strong agreement with the HEAT results.
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Alternatively, PCR was carried with 1 U of Taq-Purple polymerase and dNTPs (Top-Bio, Czech Republic).
Total RNA was extracted using RNA Blue kit (Top-Bio, Czech Republic) and the concentrations were leveled exactly to 1 µg µL−1 by DEPC-treated water in all samples.
The PCR reactions, in a volume of 50 µl, consisted of 1× La buffer, 100 µm dNTPs, 50 pm of each primer, 1U LA DNA polymerase (Top-bio), and 2 µl DNA.
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