They include a partial left mandible with teeth, a matrix with anterior skull fragments and impressions, three vertebrae, and some isolated teeth, respectively.
In tricuspid teeth, enamel matrix was deposited first on the central major cusp, then on the two adjacent minor cusps.
The functions of ameloblasts during maturation-stage tooth development include matrix turnover, calcium handling, pH regulation and ion transport [ 13].
In this context, it is worth mentioning that CUR has recently also been shown to inhibit adherence to teeth and extracellular matrix of Streptococcus mutans, the major causative agent of human caries [ 14, 15].
Moreover, ascorbic acid acting as a chain breaking antioxidant impairs with the formation of free radicals in the process of formation of intracellular substances throughout the body, including collagen, bone matrix and tooth dentine [ 42].
In addition, ascorbic acid acting as a chain-breaking antioxidant impairs the formation of free radicals during the biosynthesis of intracellular and extracellular substances throughout the body, including collagen, bone matrix and tooth dentine [ 34].
Breschi et al. [10] and Visse and Nagase [104] described MMPs as a class of zinc- and calcium-dependent endopeptidases that are trapped within the mineralized dentin matrix during tooth development [5,105,106].
Ascorbic acid is used as reference standard because ascorbic acid acts as a chain breaking scavenging agent that impairs the formation of free radicals in the process of intracellular substance formation throughout the body, including collagen, bone matrix, and tooth dentine [ 30].
This is likely due to the fact that clear aligners can be removed and, therefore, cleaned more effectively to remove dental plaque a complex community of microorganisms existing on the surface of the teeth in a polymeric matrix of bacterial origin.
