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Overall, these capabilities provide a versatile, high-throughput approach to develop tools and reagents useful for a plethora of experimental hematology applications.
Manipulation of the embryos is straightforward, and many molecular tools and reagents are available.
While for Toxoplasma there are a wide array of molecular tools and reagents available for experimental investigation, relatively few reagents exist for Neospora.
JR contributed analytical tools and reagents.
Edward Esposito, Scott Gridley and Birgit Leitinger contributed new tools and reagents.
A complete list of surgical tools and reagents used is described in detail in Table 1.
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Continued tool and reagent development is critical for the survival, expansion, and evolution of the Drosophila field.
—Ethan Bier, University of California, San Diego Continued tool and reagent development is critical for the survival, expansion, and evolution of the Drosophila field.
The motivation for implementing ZFNGenome, summarized in Figure 2, was to create a user-friendly interface between two valuable open-source genomic resources: i) established genome browsers, with associated genomic DNA sequences, annotations and other resources available for model organisms; and ii) ZFN design software tools and experimental reagents made available by the Zinc Finger Consortium.
The efficacy of TALENs across a wide variety of species, together with the availability of open-source target prediction tools, construction protocols, and reagents (available via Addgene: http://www.addgene.org/TALEN/), make this technology ideally suited for precision genome engineering in nonconventional model organisms.
JCS, ES, JS, PDG, and MCH provided reagents, tools, and ideas.
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