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This threshold we used in a customized macro (Source code 1) that utilizes the 'connected threshold grower' plugin of the ImageJ 3D toolkit to determine nuclear staining intensities in all slices of the z-stack.
Therefore, we performed a posterior power calculation using the stochastic simulation and estimation tool of the PsN toolkit to determine the power (95 and 99% confidence) of our study to find a clinically relevant genotype effect (at least 20%) on cyclosporine, everolimus, and tacrolimus PK.
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We used PLS and Nimrod toolkits to determine the most influential ion diffusion parameter(s) governing the properties of the electrical signal (such as APD60) and of the Ca2+ signal (such as peak level of Ca2+ transient in the junctional cleft, subsarcolemmal space, and bulk cytosol).
More work is needed to web-enable the toolkit, and to determine its potential predictive power.
To determine toolkit effectiveness, Lugtenberg et al's 20 method was adopted to assign outcomes from each toolkit to one of three categories: (1) not effective (if no significant effects were demonstrated); (2) partially effective (if half or less of the outcome measures showed significant effects) or (3) mostly effective (if more than half the outcome measures showed significant effects).
The toolkit can be used to determine (i) the relative risk of a farm enterprise contributing to microbial watercourse pollution and (ii) appropriate and targeted mitigation to reduce the risk of FIO loss from land to water.
One should consider what their data looks like now, as well as what data they might be working with in the future, in order to determine if a particular toolkit will be appropriate.
The toolkit provides tools that can be used to determine which educational and training interventions for health workers are effective; evidence from these tools can be used to inform scaling-up or redistribution strategies.
For the designers it was important to determine what format(s) the toolkit should take; how it could fit best and prove most useful within users' existing patterns of work and routines on site; as well as informing what level and kinds of content should be presented, in order to produce a genuinely accessible, usable and valuable toolkit for these groups.
FastX toolkit and Sickle paired-end trimmers were used to determine low quality reads towards the 3' and 5' ends of the reads.
Clearly, the powerful genetic toolkit of D. melanogaster can now be applied to determine the molecular deficits that underlie such phenotypic changes for these, and many other, lincRNA loci.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com