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BioR can also perform searches on identifiers that can be indexed using a BioR toolkit command for fast querying.
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Users of the toolkit may now script the same toolkit commands for the PubChem database as they can for a local read-only structure file.
Scripts can also be intermixed with toolkit commands as long as the TSJON format is maintained.
All microvessels were reduced to black lines (Photoshop Adobe processing toolkit (RGI) command: Filter: Erode and Skeletonize).
Using Adobe Image processing toolkit (Adobe command: Filter: IP* lines+points, total length), we were able to calculate total vessel length.
The sequential and parallel commands defined in pymicmac can be executed with pycoeman (https://github.com/NLeSC/pycoeman), a Python toolkit for executing command-line commands.
The BioR toolkit also includes commands to convert tab-delimited input file into TJSON strings (such as VCF and BED files) or convert TJSON into tab-delimited output file.
Finally, the BioR toolkit supports two command-line utilities for annotating variants: (i) bior_snpeff, which integrates SnpEff annotations (Cingolani et al., 2012), and (ii) bior_vep to annotate files using Ensemble's variant effect predictor (www.ensembl.org/info/docs/variation/vep/).org/info/docs/variation/vep/
HierS and Badapple were implemented using the ChemAxon JChem Java toolkit [41] with both command line and web interfaces.
Commands from the toolkit can be combined with other UNIX commands for advanced annotation processing.
The QsarDB Toolkit [20] contains a command-line application for defining and calculating descriptors using the CDK chemoinformatics library (see example in Additional file 1, Section 2.4).
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