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The quality of the source signals were also evaluated with the PEASS toolkit as described in section 4.2.3.
SNPs were called independently for the draft genomes BspSym, BazSymA, and BazSymB with the Genome Analysis ToolKit as described by De Wit et al. (2012) with some modifications (McKenna et al., 2010).
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Now, tongue as described.
Unpaired Single Nucleotide_Variant (SNV) calling was performed using the Genome Analysis Toolkit pipeline as described, followed by the SeattleSeq annotation and filtering steps.
We asked for what went well and what went wrong so as to assess patient satisfaction as described in the toolkit for high quality of neonatal care services [ 25].
Reads were aligned to the human reference sequence hg19 using the Burrows–Wheeler Aligner, and the Genome Analysis Toolkit was used for base recalibration, local realignment and variant calling, following published best practice guidelines, and as described previously [ 22].
as described previously [27].
In order to quantify the fluorescence signal in each individual cell, we generated an automated pipeline for image analysis as described previously (Davies et al., 2013) or used tools provided through the Farsight project (http://www.farsight-toolkit.org).org
Interlude (as described above).
Wind as described above.
(The full sequence in the toolkit can be described as 'think perpetrator', 'think opportunity'thinkintervention'ion', think designer' and 'think manager').
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