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Sampling was done using the Computer-assisted stereological toolbox system (Olympus Denmark A/S, Ballerup, Denmark), using an Olympus BX51 microscope, a motorized microscope stage (Prior Scientific, Rockland, MA) run by an IBM-compatible computer, and a microcator (ND 281B, Heidenhain, Schaumburg, IL) connected to the stage and feeding the computer with the distance information in the z-axis.
Sampling was done using the Computer assisted stereological toolbox system, version 2.1.4 (Olympus), using an Olympus BX51 microscope, a motorized microscope stage (Prior Scientific, Rockland, NY) run by an IBM compatible computer, and a microcator (Heidenhain ND 281B, Schaumberg, IL) connected to the stage and feeding the computer with the distance information in the z-axis.
The Computer-Assisted Stereological Toolbox system, version 2.1.4 (Olympus, Ballerup, Denmark) equipped with an Olympus BX51 microscope, a motorized microscope stage (Prior Scientific, Rockland, MA) run by an IBM-compatible computer, and a microcastor (ND 281B, Heidenhain, Traunreut, Germany) connected to the stage and feeding the computer with the distance information in the z-axis was used.
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Functional proteomics are an important part of the toolbox of system biology, trying to elucidate the dynamic nature of the proteome composition and complex assembly and disassembly, the absolute and relative quantitation of the parts of these complexes, and the proteome as a whole.
Stereological analysis was performed using light microscopy and the New Computer Assisted Stereology Toolbox (NEWCAST) system from Visiopharm (Hoersholm, Denmark).
Secondly, as time, place and costs are important factors to successfully adopt and implement the program [ 32, 33], the three sessions are organized within the existing so-called "Toolbox Education system" in the Dutch construction industry.
The toolbox education system consists of at least 10 obligatory health and safety training sessions for workers, which have to be organized by managers in the construction industry each year in order to obtain an official health and safety certificate.
We have implemented the proposed algorithm in Mathematica as part of a Parameter Estimation Toolbox for Systems Biology developed by the Systems Biology group at the Fraunhofer Chalmers Centre (FCC) in Göteborg (Sweden).
The ML estimations have been done using the Parameter Estimation Toolbox for Systems Biology developed by the Systems Biology group at the Fraunhofer Chalmers Centre (FCC) in Göteborg (Sweden).
We believe that the presented methodology offers an attractive alternative to other approaches and will be a useful addition to the toolbox of systems biologists.
The simulations were run using Matlab and KroneckerBio v0.3.0 alpha, a MATLAB-based toolbox for systems biology model building, simulation and analysis built by members of the Tidor laboratory.
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