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The technique was also an important tool in gene sequencing.
Two-colour microarray experiments form an important tool in gene expression analysis.
Adenovirus is the leading tool in gene therapy because of its ability to insert DNA into a variety of cells.
Although traditional linkage based QTL-mapping has become an important tool in gene tagging of crops, it has few limitations viz., 1) classical linkage mapping involves very high cost; 2) it has low resolution as it can resolve only a few alleles and 3) it has limitations towards fine mapping of QTLs as it needs BC-NILs.
In this study it was shown that the transformed DNA solely was integrated via homologous recombination in all the obtained transformants which proved that Ku deficient strain could be used as an efficient tool in gene targeting and study of gene function in A. carbonarius.
Even though this new gene editing tool is a simple, cheaper, and more precise genome editing tool providing scientists with the realisation of the long-lasting dream of gene drives, some technical hurdles still remain in using the CRISPR gene editing tool in gene drives' design (Zentner and Wade 2017).
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Custom-designed zinc finger nucleases (ZFNs) are becoming powerful tools in gene targeting the process of replacing a gene within a genome by homologous recombination.
These could be useful tools in gene therapy.
This limits the application of high-throughput molecular tools in gene discovery and cell line engineering.
Biochip microarrays have become extremely valuable tools in gene discovery, disease diagnosis, and drug discovery.
DNA microarray has been widely used as measurement tools in gene expression data analysis [ 1– 4].
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