Briefly, biofilm-forming clinical isolates of A. actinomycetemcomitans were harvested from culture plates and suspended in TSB-YE/Glc to a concentration of 108 CFU/ml [32], from which 5×108 CFU was taken and cultured in 50 ml tissue culture flasks (Cellstar #690160, Greiner Bio-One, Frickenhausen, Germany) in candle jars for 18 h at 37°C.
From a -80°C Agrobacterium stock an aliquot was taken and cultured overnight in 10 ml liquid culture medium (Luria-Bertani < LB > medium + 50 mg · L-1 kanamycin + 50 mg · L-1 rifampicin) at 28°C with constant shaking.
A single colony of strain ATCC1803 from an anaerobic cooked meat broth agar plate was taken and cultured in 5 mL of cooked meat broth medium overnight.
Afterward, samples of the bacteria on their hands was taken and cultured to see how much of the S. marcescens remained.
Biopsies from antrum and fundus were taken and cultured for H. pylori.
At autopsy, specimens from pleural fluid and the cerebral abscess were taken and cultured.
In every patient with suspected infection, blood cultures using both classical medium and Mycosis® (Becton Dickinson®) were prepared, and samples from relevant sites were also taken and cultured.
Rectal swabs (Eswab, Copan, Italy) were taken and cultured using an enrichment broth, containing cefotaxime (0.25 mg/L) and vancomycin (8 mg/L) (TSB-VC) and a selective agar plate (EbSA, Alpha-Omega, Netherlands).
For the identification of N. gonorrhoeae, cervical, anal, urethral and pharyngeal swabs were taken and cultured on preheated VCA3 agar (Biomérieux, Budapest, Hungary) and on non-selective PVX chocolate agar (Biomérieux, Budapest, Hungary).
