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For other applications such as cloning, restriction endonucleases are used to great effect, however, a restriction site may not be conveniently located, or, too many restriction sites may exist within a defined sequence.
It has been tacitly assumed that BAC clones from homoeologous chromosome regions in an allopolyploid will have too many restriction fragments in common and will be included into single contigs during contig assembly.
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"There were too many restrictions," one recalled.
"Too many restrictions," Mr. El-Hage called out.
Any interview with too many restrictions can actually do damage".
"There are already too many restrictions on reporting the courts, and this is another worrying precedent".
But they also advise donors not to place too many restrictions on future generations.
The order has raised concerns about too many restrictions on access.
Still, there is a concern that creating too many restrictions could push patients to the streets for drugs.
But available grants came with too many restrictions, and were hard to find and apply for, Mr. Provini said.
His experience is that, if you set up schemes with too many restrictions, they do not work.
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