However, 16S rRNA gene-based primers are evolutionarily too conserved among taxa to discriminate between human-specific species of Bacteroides and other closely related genera, such as Prevotella.
16S rDNA has been known to be too conserved to resolve relationships among closely related strains [24].
In common with previous SSU deep amplicon sequencing surveys of protists in marine environments [17], [18], our study revealed astonishing diversity, although SSU may be too conserved to distinguish many species of dinoflagellates [92].
Our results suggest that the barcoding fragment may be too conserved to provide unambiguous barcodes for every demosponge species, because some haplotypes in our analysis are shared by several otherwise morphologically divergent species.
Other biologically relevant GPCR residues – such as those important for structural integrity, activation, or G protein coupling – may be correlated with a limited number of partners, or may have remained too conserved during the evolution to be detected by our algorithm.
The standard (COI) barcoding fragment, amplified with the universal primers of Folmer et al. ([18]; hereafter called the M1M6 partition) is generally too conserved in diploblast phyla [19] and has led to some difficulties in resolving taxonomic and phylogeographic relationships in sponges [20], [21].
The COI gene may be too conserved to allow resolution of divergence caused by the most recent glaciation.
Because the NCR region is too conserved for evolutionary analyses, the ORF1 region of the TTV genome is most often used for genotyping.
In our covariation calculation, among the ten mutated sites, four were filtered as they were too conserved (Ile36 [ β2-1], Ile38 [ β2-3], His88 [ α2-1] and Val92 [ α2-5]).
With the very restricted phylogenetic differences found within each subtype, the chosen gene product was too conserved to allow for analysis of transmission chains.
