In other words, SNPs were observed on 11 of 12 chromosomes within the Micro-Tom lines.
AMF2 (n = 120) was derived from a cross between the 'Ailsa Craig' and 'Micro-Tom' lines, while MMF2 (n = 135) was derived from a cross between the 'M82' and 'Micro-Tom' lines.
The 30 S. lycopersicum lines included 5 'Micro-Tom' lines that were obtained from different resource centres.
Most of the polymorphic markers between the two 'Micro-Tom' lines were mapped on Chr04, Chr07, and Chr12.
In Cluster 2, small LD blocks were found on chromosomes 3, 4, 6, 7, and 12 that were caused by heterozygous regions within the five Micro-Tom lines.
The heterozygous regions in the five Micro-Tom lines might be caused by the existence of heterozygous regions in the first generation of Micro-Tom or mutations that arose during generation changes.
To address potential residual heterozygosity in the parental 'Micro-Tom' lines used to create AMF2 and MMF2, they are distinguished in this study by the designations 'Micro-Tom_AM' and 'Micro-Tom_MM', respectively.
cerasiforme line E9, S. pimpinellifolium line L5, P population individuals (F6 generation) and Micro-Tom line was isolated from leaf tissue using DNeasy 96 Plant Kit (QIAGEN).
Indeed, 'Micro-Tom' mutant lines carry mutated alleles that may confer high agricultural value to tomato, e.g. alleles causing large variations in fruit color, shape, size, and composition.
The following ALL-derived cell lines (TOM-1, NALM-20, MY, LOUCY, TANOUE and MOLT-4) were used for in vitro studies.
The methylation status of these genes was analyzed in two ALL derived cell lines (TOM-1 and NALM-20), human male genomic DNA universally methylated for all genes and peripheral blood sample of healthy donor and compare with the equivalent samples in the array.
