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The ability of TetA to broadly tolerate charge substitutions indicates that the Sec machinery assists in its insertion into the membrane.
Analogously to various substrates of ADAM 10 and 17, the cleavage of PrPC appears to tolerate charge removal or inversion.
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We thus examined how P525, another residue located between F523 and Q526, could tolerate charged residues.
In August 2001, the regime cracked down on would-be reformers, arresting prominent members of the discussion groups that it had been tolerating, charging people with "attempting to change the constitution by illegal means" and "inciting racial and sectarian strife".
Renault believes drivers who are used to paying regularly for fuel will tolerate this charge.
I524, proximal to the substrate binding residues, was the only position that did not tolerate any charged residues.
Furthermore, the only position that did not tolerate any charged residue was I524, proximal to the substrate binding residues.
However, no change in FRET efficiency was observed for V71E, from which we concluded that position 71 is sufficiently solvent-exposed that the protein can tolerate a charged side chain without a significant energy cost.
NADP+-specific dehydrogenases cannot tolerate a negatively charged residue at position 32.
It is clear, though, that Vichai and Aiyawatt were no longer willing to tolerate Pearson being in charge of their football club and that there was only one course of action in their eyes.
Charles John now had dreams of becoming king or "protector" of France, but he had become alienated from the French people, and the victorious allies would not tolerate another soldier in charge of French affairs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com