However, 'triangulation' from multiple genotypes enabled the identification of conserved and tolerant-specific responses that may provide durable tolerance across species.
However, this was a general response observed in all genotypes and is therefore not tolerant-specific.
OsSUB1B is non-responsive to GA and ethylene and no tolerant-specific allele has been identified for this gene (Xu et al., 2006).
The comparison of traditional aus-type rice with irrigated varieties allowed us to identify tolerant-specific metabolites that accumulate in shoots and/or roots under drought.
Metabolites with higher fold-change in the tolerant or intolerant genotypes, respectively, were classidified as tolerant-specific (T) or sensitive-specific (S).
For the identification of tolerant-specific metabolites, the normalised data was log10 transformed to improve normality and analysed by a two factorial ANOVA with interaction, where the factors were treatment (WW or D) and genotype.
Galactinol was identified as highly drought responsive also in this study, however, there were no significant differences between the aus-type and the irrigated varieties (Fig. 3) and galactinol is therefore not a tolerant-specific metabolite in rice.
Applying the same analysis as conducted for the annotated metabolites, we identified 17 metabolites in shoots with significant interaction between treatment and genotype, of which 16 were classified as tolerant-specific and one as sensitive-specific (Fig. 5).
Metabolites with higher fold-change in the tolerant or intolerant genotypes, respectively, were classidified as tolerant-specific (T) or sensitive-specific (S) Fig. 5 Unknown drought-responsive metabolites in tolerant and sensitive rice genotypes.
Selection of varieties that are immune, resistant to attack, or tolerant to specific pests, is a biological control procedure also widely used.
Moreover, S-PAO plants were more tolerant to specific pathogens that depend of the apoplast for their growth, like the bacteria Pseudomonas syringae and the oomycete Phytophora parasitica var.
