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Solvent tolerant microbes employ multiple resistance mechanisms.
Genetic engineering offers another means to develop highly tolerant microbes, such as in the case of Saccharomyces cerevisiae strains engineered to overexpress enzymes, transcription factors, and/or multidrug-resistance proteins that confer improved resistance to different inhibitors [ 10].
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The genomes of hydrocarbon-tolerant microbes represent a rich resource for tolerance engineering.
Based on the natural examples of solvent-tolerant microbes it is likely that integrating multiple strategies will greatly improve biofuel tolerance of a production host.
Since cold-tolerant microbes do just that in terrestrial permafrost, it is quite possible that appropriate conditions will, indeed, be found.
Salt-tolerant microbes can adapt to these conditions and degrade the organics in hyper saline wastewater.
Several pyrazine-tolerant microbes were shown to resist the treatment at lower concentrations and to increase in their relative abundance.
Solvent-tolerant microbes can actively change their membrane composition to block entry of solvents.
A combined strategy that utilizes both membrane modifications and other approaches mirrors natural examples from solvent-tolerant microbes and may prove to be an effective engineering approach.
Studies of solvent-tolerant microbes found that cells can modify fatty acid composition or other accessory molecules of their cell membrane to block the entry of solvents [ 66, 67].
Our overall goal was to identify novel biofuel tolerance genes from a hydrocarbon-tolerant microbe that could be used in E. coli.
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