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Whether in "non-tolerant" or "tolerant" groups, the effects of morphine were stronger in "obex-transected" than in "sham-operated" animals.
A follow-up study categorized the RILs into sensitive and tolerant groups and mapped the position of Saltol between RM23 and RM140 (10.7–12.2 Mb on chromosome 1), and confirmed the effect of the shoot Na K ratio with an LOD of 6.6 and R2 of 43% using 54 RILs (Bonilla et al. 2002).
In addition, known cancer genes are overrepresented in the least tolerant groups with respect to most tolerant groups.
However, genes in the least tolerant groups are significantly more conserved, on average, than genes of the most tolerant groups.
In contrast, variation at the AHR2 locus could distinguish DLC-sensitive and tolerant groups (Table 5).
Splenic B lymphocytes were separated through magnetic beads and genes were analyzed by microarray, comparing control and tolerant groups.
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THP1 differentiated into macrophages (with 2.5 nM PMA treatment) were cultivated in RPMI medium (Control group), submitted to tolerance (500 ng/ml LPS 24 hours before challenge with 1,000 ng/ml LPS - Tolerant group) and challenge (1,000 ng/ml LPS - D group) during 24 hours.
For this purpose, the tolerant aus-type varieties Dular and N22 were combined into a tolerant group and IR64 and IR74 into a sensitive group.
Metabolites were further classified depending on whether the log2FC was higher in the tolerant group or in the drought sensitive group.
In contrast, two metabolites were identified in the tolerant group in both, shoots and roots, namely uridine and 2-amino-butanoic acid (AABA), whereas raffinose was specific to the sensitive groups in shoots and roots (Fig. 4).
Conversely, several organic acids (glyceric acid, glyceric acid 3-phoshate, malic acid and citric acid), which are all components of glycolysis and the TCA cycle, were significant for roots of the tolerant group but not in shoots.
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