THP1 differentiated into macrophages (with 2.5 nM PMA treatment) were cultivated in RPMI medium (Control group), submitted to tolerance (500 ng/ml LPS 24 hours before challenge with 1,000 ng/ml LPS - Tolerant group) and challenge (1,000 ng/ml LPS - D group) during 24 hours.
Metabolites were further classified depending on whether the log2FC was higher in the tolerant group or in the drought sensitive group.
For this purpose, the tolerant aus-type varieties Dular and N22 were combined into a tolerant group and IR64 and IR74 into a sensitive group.
Conversely, several organic acids (glyceric acid, glyceric acid 3-phoshate, malic acid and citric acid), which are all components of glycolysis and the TCA cycle, were significant for roots of the tolerant group but not in shoots.
In shoots of the tolerant group, several AA (serine, methionine, asparagine, proline, threonine, arginine and its derivate ornithine) specifically accumulated in response to drought, whereas no AA showed significant interaction between treatment and genotype in roots.
In contrast, two metabolites were identified in the tolerant group in both, shoots and roots, namely uridine and 2-amino-butanoic acid (AABA), whereas raffinose was specific to the sensitive groups in shoots and roots (Fig. 4).
For the latter, Dular and N22 were combined into a tolerant group and the IR64 and IR74 into a sensitive group (Additional file 3: Table S2) which is also justified by the fact that in the PCA analysis (Fig. 2) no separation within the group of aus-type and indica type varieties could be detected.
"This doesn't sound like a very friendly, tolerant group!" Hilton remarked.
Sharing of HLA-class II was not sufficient to account for the tolerant group, as all partners showed an HLA-class II mismatch with the DR and DQ alleles, which would generate an MLR response.
If the glycine count was ≤0.505, the protein fell into the tolerant group.
If the glycine count was <0.445, the transporter fell into the tolerant group.
