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Had the rescues been due solely to phenotypic tolerance, the supposedly tolerant cells would have reverted to sensitivity in the benign environment.
Array-CGH was performed on parental and tolerant cells at the maximum tolerated PPP level including Line2, Line2-T500, Line3 and Line3-T200.
Therefore, the MIC of tolerant cells is unchanged compared with non-tolerant strains.
Often, tolerant cells are non-growing before antibiotic exposure, but not necessarily.
What is the stress es) that kill desiccation sensitive cells and how do tolerant cells mitigate these stresses to survive.
By definition, the term antibiotic persistence is always connected with a heterogeneous population, in which only a part of the population consists of tolerant cells.
Similar(39)
The genes downregulated in PPP tolerant cell lines were siRNA silenced in non-tolerant cells Line2, Line3, the melanoma cell line BE and breast cancer cell line MCF7, all known to express IGF-1R and to be responsive to PPP [8].
These results indicate that the antibiotic tolerance due to anaerobic adaptation is reversible, and this type of drug-tolerant cells can be eradicated by introducing oxygen into the environment or by using antibiotics that can kill cells at low oxygen levels.
Consequently, there are increasing numbers of reports of different types of drug-tolerant cells with different levels of metabolic activity and different patterns of drug tolerance (36, 40, 42 – 42).
Desiccation-tolerant cells implement structural, physiological and molecular mechanisms to survive severe water deficit.
Possibly, hypoxia-tolerant cells are related with mitochondrial condensed appearance and are competent to produce adequate amount of ATP by mitochondrial respiration.
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