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Search criteria were as follows: monoisotopic masses, 1 missed cleavage, tolerances set for 0.3 kDa for peptides matches, and 0.2 kDa for MS/MS fragment matches.
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Changing the mass tolerance setting for ion extraction from 10too 5 to 2 ppm did not alter the peak area of the analyte (Supporting Information, Figure S4).
The fidelity of the calibration model becomes particularly important where stringent tolerances are set, for example construction experiments or surface measurement.
In this work we use a Taylor series method of order 15 with an error tolerance set to TOL = 10 − 12 for the most of the simulations.
Screening the 71 strains of the SGRP strain set for tolerance to 1.5 mg/mL furfural identified four strains of S. cerevisiae and one strain of S. paradoxus that appeared to have increased tolerance to furfural.
Finally, the tolerance of skin hue is set for E H = ± 25 and the range of E S = ± 30 is for saturation testing based on our experiments.
Our initial candidate set for IL-tolerance screening included all 21 enzymes that showed activity at 80 or 90 °C.
In this model, a defect in central tolerance sets the stage for the eventual development of a chronic inflammatory disease.
Search tolerances were set to 5 ppm for MS and 0.5 Da for MS/MS.
Search tolerances were set to ±1.5 ppm for the mass and ±1.5% for the elution time.
Mass tolerances were set to 100 ppm for PMF searches and to 100 ppm (precursor ions) and 0.7 Da (fragment ions) for MS/MS ion searches.
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CEO of Professional Science Editing for Scientists @ prosciediting.com