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MS1 tolerance was 10 p.p.m. and MS2 tolerance was set at 0.8 Da.
Band positions were assigned manually, with computer assistance, and the band tolerance was set at 2%.
Mass tolerance was set at 20 p.p.m. for intact peptide mass and 0.5 Da for fragment ions.
Maximum missing cleavage was set at 3, and mass tolerance was set at ±0.01 Da for precursor ions and ±0.5 Da for MS/MS.
Maximum missing cleavage was set at 4, and mass tolerance was set at 10 ppm for precursor ions and ±0.05 Da for MS/MS.
The error tolerance was set at 100 p.p.m (ESI-QTOF).
Similar(10)
The precursor and fragment mass tolerance were set at 20 ppm and 0.04 Da, respectively.
Peptide mass tolerance and fragment mass tolerance were set at 0.8 Dalton.
For each RSP, the convergence tolerance is set at 10−5 and the maximum iteration number is set at 300.
Both the precursor mass tolerance and the MS/MS tolerance were set at 30 ppm and 0.35 Da, respectively, allowing 1 missed tryptic cleavage site.
The optimization and tolerance were set at 1.00%.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com