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These rare exceptions could be due to mis-sequencing of the genome or to tolerance of evolutionary changes in the species.
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We observed that genes classified as specific to the embryonic gonadal category were involved in significantly more interactions than those specific to the adult gonads, suggesting that lack of pleiotropy-mediated constraint may play some role in explaining the tolerance for evolutionary divergence of adult gonad specific genes when compared to those of other tissues and stages.
High levels of stress tolerance are the result of evolutionary adaptation.
In the present study, we used a xylose utilizing recombinant S. cerevisiae[ 26] as the initial strain to enhance inhibitor tolerance in two modes of evolutionary engineering, one in repetitive batch cultures of defined medium containing a cocktail of inhibitors that are constituent of spruce hydrolysate and another in chemostat cultures using spruce hydrolysate.
In plants, the conserved C-repeat binding factor (CBF) pathway has been associated to low temperature tolerance in a wide range of evolutionary distinct species [ 33].
The previously underappreciated difference in the tolerance of gene duplications and contrasting evolutionary modes of "single-copy control" versus "multicopy license" may reflect a major evolutionary mechanism that allows extended exploration of gene sequence space.
Thus, cold tolerance plasticity may be a by-product of evolutionary history.
In the 22 family subtrees analyzed, we observed a range of evolutionary patterns of salt tolerance (see Fig. 1 and Additional file 1: Figure S1).
We propose that this baseline tolerance can complement the evaluation of violations of evolutionary constraints imposed on individual amino acid residues by protein structure and function.
In recent years, the advancement of tolerance engineering and evolutionary engineering provides powerful platform for obtaining host strains with desired tolerance for further metabolic engineering of biofuel pathways.
The maquette approach to protein design offers precisely this, employing simple four-α-helix bundle scaffolds devoid of evolutionary complexity and with proven tolerance towards iterative protein engineering.
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