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To remain practical, the described method employs a two-step registration strategy designed to reduce computation time: the first step computes a B-spline deformable transform on low-resolution images prior to visualization, the second step applies the precomputed transformation only to the high-resolution region currently being viewed.
Prior to visualization, the overall network size was reduced in two ways.
Prior to visualization, the PCR products were denatured at 95°C for 10 minutes.
Prior to visualization, the blot was washed in PBS/0.1% Tween (3 × 5 min) and once more in PBS.
Prior to visualization, the cells were washed with PBS and fixed with 3.7% formaldehyde for 20 min at room temperature.
Prior to visualization, the nucleus of the cells were stained with a nuclear dye 4′,6′ diamino-2-phenylindole (DAPI).
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Here, in situ visualization refers to visualization of the image data on the movable FRISM display, while ex situ refers to visualization on a fixed conventional display.
The particles were washed several times prior to visualization under the fluorescent microscope.
Heating of the plates to 160°C for 10 min led to visualization of the separated lipids.
After vertebral ossification, ultrasound is limited to visualization of the spinous processes and interspinous spaces.
Diaminobenzidine (DAB, Dako) was applied for 5 minutes to visualization of the reaction product.
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