We used quantitative real-time PCR to verify the expression of the differentially expressed genes in wild-type tissues following the transition to SD treatment.
We used quantitative RT-PCR to verify the expression of candidate genes from two general categories: genes classified as over-expressed in the NDI and DI treatments from the TMM-normalized 454 analysis, and genes that were of a priori interest based on their role in the diapause response in other insects (See additional file 6: Results of qPCR validation).
The methods to verify the expression of cartilage-specific molecules usually involve destructive procedures to recover the cells from the scaffolds for tests.
The aim of this study was to identify the sequence of NPR-B in Sus Scrofa, which is not present in GenBank, to verify the expression of NPR-B in the different cardiac chambers of normal pigs and evaluate its homology with murine and human species.
To understand the increase in mannitol- and drought-stress tolerance of transgenic OsADF3 OE Arabidopsis, we used real-time PCR analysis to verify the expression of these genes.
To verify the expression of DR6 on pDCs at the protein level, we used αDR6, homemade DQM3 (Hu et al., 2014), to stain fresh human PBMC.
Quantitative PCR (qPCR) was performed to verify the expression of breast cancer-related genes (ERα, ErbB2, progesterone receptor (PR), and BRCA1) in each tumor cell lines.
METHODS: To verify the expression of ERs in fibromatosis as well as to clarify the inconsistency between radioligand and early immunohistochemical studies, the authors examined a series of 40 extraabdominal fibromatoses using antibodies raised against ERbeta.
In order to verify the expression of estrogen receptors in vascular neoplasms as well as to clarify the inconsistency between radioligand and early immunohistochemical studies, we examined a series of 53 benign and malignant vascular neoplasms for ER beta expression.
In order to verify the expression of the alkB gene cluster under the control of the Ptip/regulator system, experiments with resting cells of Rhodococcus erytropolis AP pTipQT1-alk R7) exposed to C12 were performed.
Western blot was performed to verify the expression of GADD153.
