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Those differentiated markers need to validated with double or triple co-staining.
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To obtain reliable data we recommend to validated all (possible) HAI with the NH physician and at least one ICP or other dedicated and trained professional.
It was acknowledged that there is an urgent need to share platforms (like eHealth), compatible with comprehensive geriatric assessments (BelRAI, a Belgian version of the InterRAI) [ 29], with links to validated guidelines for Belgium (EBMPracticeNet) [ 28], and with high level security access and the necessary legal privacy clearances.
An improvement in the implementation of validated methods could arise from a better coordination on the matter at national and international levels, the harmonisation of different legislations, and a strict control of the national rules in order to make them up-to-date with respect to validated methods.
Additionally, we assessed all methods and a ninth one with respect to validated experimental data.
This provides strong evidence that the original result – TEs exapted in 4% of the human proteome – is roughly off by an order of magnitude, when examined with respect to validated protein sequences as opposed to predictions of protein sequences.
Especially PaD213-A5 exhibits a highly interesting property of differentiating between Aβ42 fibrils based on their conformation that is not yet described in literature and its implication on AD diagnosis and therapy has to be further validated with in vivo data.
Sample collection and analysis of microdialysates and blood samples by capillary electrophoresis were performed according to a validated method with minor modifications (Mader et al, 1998).
Briefly, all F1 and F2 individuals were genotyped for a set of 24 unlinked microsatellite markers with individuals assigned to parent pairs using maximum-likelihood methods implemented in CERVUS v3.0 [ 44] and further validated with reference to major histocompatibility complex (MHC) and major urinary protein (MUP) genotyping [ 43].
It has been thoroughly validated with regard to foods and nutrients (Brantsaeter et al. 2008a).
Both assays performances were thoroughly validated with regard to recovery, linearity in plasma dilutions, and intra- and inter-variation.
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