Quantitative RT-PCR was used to validate the expression of select differentially expressed miRNAs bta-miR-199a-3p bta-miR-199a-3p bta-miR-199a-3p bta-miR-199a-3p
A quantitative real-time RT-PCR approach was used to validate the expression of 10 genes identified as differentially expressed in the microarray analysis, including 7 up- and 3 down-regulated genes.
Quantitative RT-PCR was performed to validate the expression of selected genes identified as differentially expressed between the two CC groups by using mRNAs from HP-hMG (n = 4) and rFSH (n = 4) CC samples as described in [ 30].
To confirm the differential expression pattern, we performed qRT-PCR to validate the expression of miR-126, miR-222, and miR-345 in the validation cohort samples with or without CNS relapse (N = 3 and N = 26, respectively) as shown in Figure 2D.
Real time PCR was used to validate the expression of three genes in each of liver and muscle that were differently expressed in both the Sept. and Dec. lots.
Quantitative real-time reverse transcription PCR was also performed to validate the expression of these genes after heat stress.
In this study, we designed RT-PCR primers using conserved 5′ regions and the mouse 3′ domain to validate the expression of Vamp1.
Availability of common SSR markers across the species provides an opportunity to validate the expression of QTLs across variable genetic backgrounds and accurately compare the position of QTLs in other species.
RT-qPCR was used to validate the expression of 11 selected genes from the microarray analysis.
We chose to validate the expression of several miRNAs during senescence with quantitative real-time PCR.
To validate the expression of the lacZ marker from the ViaatlacZ and Gad1lacZ knockin alleles, we performed an initial analysis of β-galactosidase expression in the knockin mice.
