This capability was further used to validate specific molecular dynamics algorithms and to identify those that resulted in over-estimate or under-estimate of the magnitude of protein motions.
Competition winners receive the opportunity to interview for jobs as well as certifications to validate specific skill sets.
However, in retrospect the use of a different P. aeruginosa strain background to validate the results with specific Tn mutants based on transcriptome-findings with a heterologous strain probably adds robustness to the data as strain-specific effects are less likely to have been analyzed in the follow-up studies with the Tn mutants.
To validate the results pertaining to specific genes, we manually inspected multiple sequence alignments and interrogated raw bowhead whale transcriptome data for selected genes.
To validate our results as target-specific, compound 1 was assessed in a range of cytotoxic, anti-parasitic, and anti-microbial assays.
rSNP-MAPPER can therefore be used to generate hypotheses on potential regulatory SNPs, by screening all SNPs in a genomic region of interest, or to validate experimental results, by analyzing a specific set of SNPs.
To validate these results, we undertook a more specific analysis and conducted in vivo experiments to assess the biological relevance of the five HU regulated clusters.
To validate these results QRT-PCR with human-specific primers was performed for 11 genes of interest (hVEGFA, hCREBZF, hLTBP3, hIL4R, hIL7R, hSOS1, hSOS2, hSMAD3, hSMAD7, hSMURF2 and hTGFBR2).
Microarray experiments with RNA from whole filaments were used to validate the results of the experiments performed with cell-specific RNA.
In order to validate the microarray results, gene specific primers were designed for 13 differentially expressed T. urticae genes (7 up- and 6 down-regulated genes) using Primer 3 v0.4.0.
To validate sequence results of the TATA box regions, single nucleotide polymorphism (SNP) specific primers were used in a fluorescence/gel-migration assay.
