Primer pR(3+4) consisted of two arms: the P4 arm was complementary to the insert and P3 arm annealed to the 3' sequence of insertion site.
Primer pF(1+2) was composed of two parts: The P2 primer was complementary to the insert and the P1 primer was identical to the 5' sequence of pre-selected insertion site.
Go to the Insert menu, and then Break, and select Section Break (Next Page).
Another option is to go to the Insert menu, choose Symbol and select the appropriate character.
DC bias was applied to the insert with the range between 0 and −120 V.
The genetic load was first divided due to the insert size.
After the 15,000 shot injection molding test, all of the coatings were still attached to the insert.
Application of Finite Element Method is used to assess the critical spots leading to the insert holder failure.
Particularly, a high fiber volume fraction next to the insert raises the risk of a formation of dry spots close to the embedded insert.
A selection of PMMA plates, 3 mm thick, were designed, containing drilled holes to hold the micro-tubes, which attach directly to the insert (Fig. 2).
On the other hand, on porous scaffold, the cells adhered with their whole surface to the insert penetrating the porous structure, while on the polished surface, more spherical cells were observed with a smaller surface of adhesion.
