To analyse whether downstream factors of Id-1 are responsible for its pro-osteolytic effect in prostate cancer cells, we used RT PCR to test the expression of several secretory factors in prostate cancer cells expressing various levels of Id-1.
Quantitative real-time PCR was performed to test the expression of HIF-1α and VEGF1 in HUVECs as well as IL-1β and TNF-α in RAW264.7 cell line.
Dual-luciferase reporter assay, real-time quantitative PCR (qRT-PCR) and related phenotypic experiments were used to test the expression of related genes and the therapeutic effects of our devices.
The method of immunohistochemical is utilized to test the expression of CXCR7 and NF-κB in the breast cancer tissue, compared with the para-carcinoma tissue, and analyze its relevance with the clinicopathologic features of the breast cancer tissue, such as tumor size, TNM staging, lymphatic metastasis and other conditions.
Both Western blot and immunohistochemical staining analyses were used to test the expression of Hpa in HepG2, SGC-7901, MKN45, MCF-7, SW480, and U2OS cells.
Quantitative RT-PCR was performed to test the expression of some drought- and salt-responsive genes in ABA-dependent signal transduction such as RD29B (Yamaguchi-Shinozaki and Shinozaki 1994), RAB18 (Lang and Palva 1992) and KIN2 (Gilmour et al. 1992; Kurkela and Borg-Franck 1992) after the plants had been subjected to dehydration or salt treatment for 6 h, respectively (Fig. 9).
In addition to this peripheral protein, we also decided to test the expression of five intrinsic Arabidopsis membrane proteins.
Primer sets used to test the expression of the two forms of CTIP2 were designed using Primer3 Software.
hIGF2exon6Fw and hIGF2exon7R were used with universal probelibrary 63 to test the expression of P2 P4 derived IGF2 transcripts.
This observation allowed us to test the expression of Rae-1δ or Rae-1ε transcripts under the control of promoter 2 alone, which we called short transcripts.
To test the expression of the iron uptake system, yersiniabactin production was tested using a GFP-reporter assay [2], [40], [57].
