Also note that "replacement" in this context refers to subsequent samples; any one point may only be sampled once per iteration in this procedure to mimic Ratcliffe (2004).
After injecting 8 µl (4.5 µg) of sample, the column was washed for 10 min (at 10 µl/min) with 2% B, and the peptides were then eluted (at 10 µl/min) using the following gradient: 2 70% B over 136 min, 70 95% B over 1 min, and 95% B for 15 min. The column was re-equilibrated with 2% B for 30 min prior to subsequent sample loading.
In order to investigate the effect of treatment group on owners' responses to questionnaire items, we first looked at the change in response from Time 1 to subsequent sample times.
Precise parameter values will result in more reliable model simulations, which can be used for interpretation, or can in turn serve to plan subsequent sampling campaigns to further constrain the model parameters.
This could help to narrow down which part of the distribution is genetically most similar to populations in question, and could help to focus subsequent sampling.
At very long intervals between sample points, little information is collected about each trajectory, such that, in the extreme case, fixations are observed as changes in frequency from 0 to 1 at subsequent sample points.
The rat organs were first freeze-dried to minimize and to facilitate subsequent sample preparation steps.
For each cell type, at least three biological replicates, corresponding to approximately 10 cells each, were prepared and subjected to the subsequent sample processing steps.
Care was taken to collect subsequent samples from same locations.
The cDNA was purified, size selected and each sample was differentially barcoded before amplification to allow subsequent sample identification.
