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Luckily, I came across that realisation at an early stage when studying leukaemia stem cells, which brought me back to studying the functions of the immune system.
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In the past, large efforts have been devoted to studying the function of individual genes and static properties of biological pathways.
Our strategy utilizing relatively accessible techniques and tools should be applicable to studying the function of other transcription factors.
In addition to studying the function of genes within the nervous system, the Gal4/UAS system is well suited to studying neural circuit function.
Mice with deleted genes are commonly produced to study the functions of genes, by seeing what happens when the gene is missing.
Targeted mutagenesis in mice has been used to study the functions of Mecps in vivo.
To study the functions of FLNa we established HT-1080 human fibrosarcoma cell lines lacking FLNa expression.
To study the functions of non-essential genes, growth rates (fitness) of the S. cerevisiae deletion strains have been examined in various culture conditions [8] [10].
Therefore, filopodia of Drosophila growth cones provide suitable readouts to study the functions of actin regulators [13], [14], and these regulators are evolutionarily well conserved [15].
To screen genes controlling architecture in rice, we used a reverse genetics approach to study the functions of transcript-factor genes using a microarray containing 10K cDNAs [25].
To study the functions of these interfaces we designed mutations that would disrupt them and determined how the mutations affected assembly and GTP hydrolysis.
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