Sentence examples for to specified stages from inspiring English sources

Embryos were allowed to develop to specified stages, harvested in phosphate buffered saline (PBS), monitored for GFP fluorescence and fixed in paraformaldehyde (PFA, 4% in PBS).

These data are supplemented with estimates of their annotation and relative expression levels at specified stages of ingrowth wall construction along with noting whether their homologues have been detected in transcriptomes of BETCs and nematode giant TCs (Additional file 5).

For examination of Notch target expression, total RNA was isolated from wild-type and mutant fins and larvae at the specified stages using TRIZOL according to standard protocols.

We found only subtle (tough still detectable) differences in endopolyploidy levels (EI) of the floral organs between specified stages of the flower development.

Tg[fli-EGFP] transgenic larvae at the specified stages were visualized and imaged live or were fixed in 4% PFA and GFP signals were detected using an anti-GFP antibody.

The stage load applications value, W t), for a specified stage period (t) is obtained from Eq. 2 using the design load applications level, W(T), estimated from conducting appropriate traffic studies.

The plausible mechanisms for the reduced cdx-2 expression in carcinogenesis, could be that homeodomain proteins signifies roles in directing the cells to specified cell-phenotype during organogenesis in early stages of development.

In the second stage, regression models are postulated to ascertain how the baseline and rate of change of cartilage volume parameters from the first-stage model vary according to specified factors/covariates [ 15].

By contrast, a powerful celebrity would seek to limit the moral turpitude clause to specified acts.

task_info - Return per-task information according to specified flavor.

The directives provide for a network of member state designated conservation areas across Europe relating to specified habitats and birds.