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It seems to be widely accepted that a nano-scale refers to sizes between 0.001 and 0.1 μm (1 to 100 nm).
NaCl porogens sieved to sizes between 10 32 and 250 425 μm or purchased commercially (1800 μm, Sigma-Aldrich) were added to the solution.
The selected poly-A RNA was subjected to fragmentation to sizes between 100 and 300 nt by incubating in fragmentation mix for 8 min at 94°C.
Because of the large number of cows without mastitis, the datasets were reduced to sizes between 1/6 and 2/5 of the original data.
Total cellular DNA was sheared to sizes between 200 and 800 base pairs by using a Covaris S2 sonicator according to the Illumina TruSeq DNA protocol and then libraries were prepared using the Illumina TruSeq™DNA Sample Prep Kit on Tecan Freedom EVO 200 Automated Liquid Handling System.
Similar(55)
These corresponded to size differences between the two alleles of 45, 84, 99, 126 and 2193 bp, respectively.
Potential errors due to size shifts between the inhaled and exhaled samples and coagulation were simulated.
The amplified products are then separated according to size differences between the adaptors.
The presence of different sized tandem repeats in the A+T-rich region leads to size variation between their mitochondrial genomes.
Thus, recombination-based mapping will not provide the necessary coverage and resolution to anchor a physical map and to estimate gap sizes between contigs.
The BBD allowed to obtain nanoparticle sizes between 10 and 32 nm.
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