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Fixed cells were spotted onto glow discharged formvar-carbon coated 300 mesh copper grids (Electron Microscopy Sciences), and allowed to settled for 1 min. Grids were then washed with two drops of mQ water, stained for 15 seconds with 1% uranyl acetate, washed again with two drops of mQ water and air dried.
Mononuclear cells (PBMCs) were prepared from diluted peripheral blood (1 2 in Hanks Balanced Salt Solution), which was layered over Histopaque 1077 (Sigma, St . Louis MO, USA) solution, centrifuged (400 g), washed and suspended in D-MEM/10% FCS. 3 × 106 PBMCs/cm2 were plated in 24-well plates or in chamber slides and allowed to settled for 2 hours.
Similar(56)
"We had to settle for sport".
Teddy Tamgho had to settle for the bronze.
He had to settle for explaining life".
PAGE A10 Lawyers to Settle for Less?
Do we have to settle for Colgate?
He had to settle for the record.
Migliore had to settle for second.
He had to settle for bronze.
He might have to settle for bronze.
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