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This rapid, simple, economical, and highly sensitive assay provides a practical alternative to sequencing for genotypic evaluation of RIF resistance in Mycobacterium tuberculosis.
These findings support the validity of the optimized CE approach as a discriminatory and time- and cost-saving alternative to sequencing for identification of Cryptosporidium species and GP60 alleles in domestic ruminants.
All of the PCR products were subjected to sequencing for confirmation.
Comprehensive ATM mutation screening of all coding exons and splice junctions has been carried out using the DOVAM-S [23], [24] method, which is comparable to sequencing for mutation detection.
Several alternative methods are available that enrich target regions prior to sequencing (for review, see Mamanova et al. 2010 ).
PCR products were linked to pEasy-blunt vector and 8 to 9 positive cloned were sent to sequencing for each product.
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Ancient genetic information has been extremely difficult to sequence, for at least two reasons.
MAWs that are not present in gene models may correspond to sequences for splice events that are unknown.
In Sanger sequencing, the underlying sequence can give rise to secondary structure which can be difficult to sequence, for example in GC-rich regions.
Primer sequences correspond to sequences for human cDNAs deposited to GenBank.
Probes were assigned to sequences for BLASTN alignments with >90% coverage and >95% sequence identity.
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