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Three fragments of 220 bp, 406 bp, and 2.8 kbp were obtained in restriction digestion of plasmids pJET-GCGN, whereas plasmids pJET-BBCGN upon Pst I digestion yielded 261 bp, 566 bp, and 2.6 kbp fragments due to reverse orientation.
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The depicted Wdnm1-like sequence maps within Ensembl database ChlSab1.0, Chr.16 positions 29807079-to-29810262, reverse orientation.
The depicted horse Wdnm1-like sequence maps within Ensembl database EquCab2, Chr.11 positions 36986601-to-36989473, reverse orientation.
The depicted chimpanzee Wdnm1-like sequence maps within Ensembl database CHIMP2.1.4, Chr.17 positions 588018940-to-58805072, reverse orientation.
The depicted olive Wdnm1-like sequence maps within Ensembl database Panu_2.0, scaffold JH685681 positions 60156-to-64601, reverse orientation.
The depicted tarsier Wdnm1-like sequence maps within Ensembl database tarSyr1, scaffold_1716 positions 51738-to-55873, reverse orientation.
In addition, we created reverse orientation constructs for each insert to verify orientation independence.
means direct orientation; - reverse orientation of the AuxREP&S elements.
Sequences were searched in forward and reverse orientation.
PCR products were sequenced in both forward and reverse orientation.
F and R indicate forward and reverse orientation.
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