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These may contain misassemblies or require further 'finishing' work to resolve gaps (Chain et al., 2009).
Genomes were finished by using PCR and traditional dideoxynucleotide sequencing (Sanger) to resolve gaps and repetitive and low coverage areas.
Sanger sequencing of PCR products and local reassembly were used to resolve gaps, misassemblies and unclear regions.
Roche 454 reads now exceed 700 base pairs in length and thus can be used to resolve gaps caused by small repeats.
Paired-end reads that were used to resolve gaps were verified by checking the position and orientation of their downstream mate.
Furthermore, we demonstrate how the model can be used to reconcile existing virulence data and to resolve gaps in our understanding of pathogen metabolism and host-cell nutrient environment.
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Consequently, we used the GapFind and GapFill [ 34] algorithms to computationally identify and resolve gaps, thus minimizing the amount of manual curation needed.
Our effort to arrange contigs into a high quality assembly and resolve gaps present in the scaffolds benefitted greatly from the availability of genome (optical) maps.
In order to be able undertake their roles, research managers need a range of capacities [ 11, 12], including being able to: establish and manage some of the research interface mechanisms described below; communicate and broker different R&D needs and findings; and identify and resolve gaps and conflicts between groups, and synthesise research perspectives.
Thus the smallest gaps in our phantom that we would expect to resolve are gaps 4 (44.2 μm) and 2 (55.7 μm), respectively.
Work is ongoing to resolve the gaps in the genome assembly of chromosome 5.
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