These data show that for the system described here to be useful in mosquito stages in vivo, either its genetic components or the delivery of ATc to the mosquito require further optimization, but that in liver stages ran-TRAD4-Sc provides an effective means of regulating expression of a transgene.
The differentiation is a must because its ranking algorithms require further optimization to be able to correctly float important simple/status tweets.
A third explanation for the low counts by FACS is that the method of mixing protoplasts with CPNs may require further optimization to ensure a broader population is exposed to CPNs.
Although S-adenosylmethionine competitive inhibitors have demonstrated pharmacological proof-of-principle in MLLr-leukemia, these compounds require further optimization to improve cellular potency and pharmacokinetic stability.
Although the speB qPCR had excellent sensitivity and specificity, this assay would require further optimization to be used as a rapid diagnostic tool given the current lengthy DNA extraction protocol (optimized to maximize DNA yields).
Metabolically competent cell lines, precursor cells and stem cells feature prominently as the most promising models for investigating diseases, toxicity and metabolic functions, although these will require further optimization to represent complete replacements for primary hepatocytes.
Despite the fact that the currently available mimic chemistries require further optimization to increase stability and efficacy, our data clearly support the notion that miRNA mimics can be used to therapeutically increase miRNA levels and that miR-29 is a potent therapeutic miRNA for treating pulmonary fibrosis.
Many of these drugs clearly require further optimization or repurposing to maximize their potential impact beyond the limited scope of their original development programes.
However, the process is difficult to control, and treatment rates and digestion efficiency require further optimization.
These data show that while this compound does appear to be specifically targeting the desired trypanosome kinase and appears to have little effect on the human kinome, it lacks sufficient potency to achieve the desired trypanocidal effect and would require further optimization.
Certain challenging pathogens and weak immune responder subjects may require further optimization of the adjuvant formulation to maximize the magnitude and duration of the protective immunity.
