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The regression defined in Equation 1 was then used to remove variations in foF2 related to the solar activity effect, allowing monthly absolute deviations (defined as ∆foF2 = foF2 - foF2′), which can potentially reveal long-term trends in foF2, to be calculated (Figure 2b).
Following alignment, the recovered miRNA counts need to be normalized to remove variations in the data that are of non-biological origins and can affect the measured abundance levels.
To remove variations of paralogous reads and variations between Williams 82 and Enrei, another SNV set was collected for each plant by using the same modules with the following relaxed parameters: non-specific matches and broken read pairs included, minimum read coverage two, variant probability 10%%, and maximum expected variants four.
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Partek® Genomics Suite™ 6.4 (Partek Inc., St . Louis MO) was used to remove variation in the data due to batch effects.
We did this to remove variation due to exploratory behaviour and to allow a meaningful comparison of performances between standard and visually-restricted string pulling.
A Gaussian filter 0.2 micrometer wide was first used to remove variation in local intensities smaller than spots of single mRNAs, see Figure 7B.
Using a novel image processing method which is capable of directly analyzing worm images, we found that bright worms (after normalization to remove variation between bright and dim worms) had expression patterns that correlated with other bright worms but that dim worms fell into two distinct expression patterns.
This linear scaling correction served to remove variation due to experimental factors in array processing.
Each alignment was cropped to remove variation on either end and then sequences were concatenated.
Furthermore, data were normalized to remove variation between subjects as a result of differences in initial MFCV.
Before analysis, we used orthogonal signal correction filtering (Wold et al. 1998) to remove variation not linked to the treatment (physiological, experimental, or instrumental variation).
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