To remove the crystal packing interaction of phosphorylated Ser158, we prepared another peptide, referred to as p-BH3BIM R154S/I155R/E158S), p-BH3BIM R154S/I155R/E158Sal substitution of R154S in addition to the I155R and E158S substitutions.
Natural selection can initially fixate enzymes or miRNAs with few targets, and then, more targets are added to the pool by the evolutionary inertness of the sequences, with natural selection only acting to remove the most deleterious of the interactions.
First, we replaced Arg 517 with alanine to remove the side-chain interactions seen in crystal structures of wild-type pol λ (supplementary Fig 1 online).
For our second step we perform crude filtration to remove the most unlikely regulatory interactions given our knowledge of gene knock-outs and knock-downs.
In order to increase the accuracy of the significance subnetwork, in our study, simulated annealing algorithm based on edge scores was applied to removing the weak interactions and enhancing the strong interactions of the calculated significance subnetwork.
Conversely, if we remove the interactions that are active in all phases of the cell cycle (25% of interactions to transcription factors), the LSCC would have only three nodes and 5 edges, many fewer than expected.
We removed the interaction and each of the two main effects and chose the best model according to AICc.
The major changes in resistance and conductivity reported by Dr. Zettl's group came about as oxygen was added or removed because the interaction of the nanotube with the gas molecules led to an electrical effect that was easily detected in a circuit.
ApoG2, a new derivative of gossypol, in which the two aldehyde groups of gossypol are completely removed, interrupts the interaction of Beclin-1 and Bcl-2/xL but not Mcl-1.
As above, this suggests to remove an interaction, namely the regulation of GAL80 by Swi5.
