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Murphy stressed the league does not want to do anything to remove hitting from the game, but that the embellishing would be continually monitored.
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This returned 1117 unique geneIDs, which were then filtered against known NifD/E and NifK/N sequences (Additional file 2: Table S3) to remove hits to conventional nitrogenase.
The flanking sequences were then blasted against LA 1589 sequences for deletion or Heinz 1706 sequences for insertion using local BLASTall with an E-value of e−20 to remove hits with low similarity.
However, most of the removed hits belonged to the identifications reported by only one algorithm (see Additional file 2).
We further curated the data to choose only unique hits and removed hits exhibiting identical 5′ and 3′ ends.
The BLAST result matrix was pruned by removing hits linking overlapping SLSs, and subsequently fed to MCL to produce a set of clusters.
Selected sequences were filtered to eliminate off-target effects by performing a BLAST search of the NCBI RefSeq database and removing hits with >15 matched bases of the anti-sense strand.
Informative BLASTP hits were identified by removing hits containing the key words predicted, hypothetical, related, and scaffold.
Therefore, BLAST results were limited to only remove hits in the sense (protein-coding) orientation.
Swisher was one player affected by the heat Thursday night, when he was removed after hitting in the eighth inning.
Prime Minister Ahmet Davutoglu said the airstrikes Friday had "removed potential threats" to Turkey, hitting their targets with "100percentt accuracy".
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