Your English writing platform
Discover LudwigExact(5)
Inaccuracies in measuring the amount of DNA in a sample will compromise normalization in pools: any variation in the amount of DNA contributed by each plant in the pool will lead to reduced representation of one or more plants.
Even though the method is optimized for Infinium 450K data, we expect that it can be applied to reduced representation bisulphite sequencing (RRBS) data or other sequencing-based approaches by fine-tuning the parameters for clustering CpGs.
Consequently, subgenomes C and D were expected to have the largest sequence divergence to F. vesca based on SSR data, and were thus expected to have a lower proportion of their re-sequenced fragments aligned to the reference genome sequence, which would lead to reduced representation of corresponding SNP markers.
We also compared the Infinium 450K data to reduced representation bisulfite sequencing data generated on the same samples and found a high level of concordance between the two independent methodologies, which can be further improved by filtering for probe sequence identity and mismatch location.
Potential bias resulting from the "healthy cohort" effect, if it is present, generally leads to more conservative results due to reduced representation of population groups who have more extreme health behaviours, such as those who are mentally or physically ill or marginalised for some other reason.
Similar(55)
The genome of S. mansoni is relatively large (364.5 Mb), and comprises ~40% repetitive sequences, so we sought to use reduced representation sequencing to minimize cost and maximize read depth in F2 progeny pools.
For organisms with a reference genome, the RIG workflow stands as a useful analysis alternative applicable to both reduced representation and WGS data, and RIG is also readily applicable to exome and RNA-seq data due to the GATK's flexibility.
By mapping to the reduced representation genome, BS-Seeker2 is more efficient and accurate than mapping to the whole genome.
To identify lineage-specific (LS) indels, we applied a Sankoff-like palgorithmalgorithm [40] to a reduced representation of each indel region (IR) in the multiple alignments.
In soybean, next generation sequencing was used to sequence a reduced representation library to find large number of SNPs and 1,790 SNPs used to construct a high resolution genetic map that allowed the assembly of 97% sequence scaffolds into a genome sequence [ 16].
Once all the relevant GO annotations were obtained, these were mapped to GOslim Plants to achieve a reduced representation of GO terms.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com