Exact(4)
It minimised the variations, secondary to read alignment and counting, by employing the same spliced aligner (Tophat2) [ 16] and counting algorithm (HTSeq 0.5.4) [ 17] for all methods, except for Cuffdiff2 that requires a unique quantification method, Cufflinks [ 18].
For detailed inspection of the hits, WebScipio generates an easy to read alignment of the query and the genome.
As with CAST simulated reads, polymorphic genes with closely related gene family members or retrotransposed pseudogenes are most sensitive to read alignment errors.
We took a conservative approach to read alignment, requiring read pairs to map perfectly and uniquely to the P. falciparum 3D7 reference genome.
Similar(56)
Once CCS reads are sorted, the pipeline uses Bowtie2's default alignment (end-to-end read alignment) to align reads to the corresponding reference sequence and outputs a set of alignments in SAM format.
In the case of sample V4528, Mosaik failed to report read alignment to the consensus.
TASR may be used in a manner that is complementary to NGS read alignment tools, in order to help confirm false-positive events that may result from NGS sequencing or mapping errors.
Of perhaps equal importance, the algorithm can be used to refine read alignment once a read has been heuristically mapped to a particular genomic location that is expected to contain an SV.
Novel transcripts predicted by ACE are commonly supported by spliced RNA-seq reads, and can be used to improve read alignment and transcript quantification when an individual-specific genome sequence is available.
The sampled numbers were regarded to be read alignment positions.
Therefore, terminal polyA/T sequences were removed from the reads using Prinseq [ 40] to improve read alignment.
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