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If the rosette test is positive, a Kleihauer Betke test is used to quantitate the number of fetal cells in the maternal circulation.
In order to quantitate the number of cells surviving in the device and optimize initial conditions leading to high-density survival, we implant devices containing C2C12 mouse myoblasts expressing a luciferase reporter in the mouse subcutaneous tissue.
To quantitate the number of stained cells, a 300×300 pixel section was removed from the region representing the arcuate nucleus in each image.
We first used indirect immunofluorescence microscopy to quantitate the number of cells that show p65 translocation from the cytoplasm into the nucleus in response to the three different types of exosomes; the fraction of cells with p65 in the nucleus is a sensitive measure of NF-κB activation [31].
AnnexinV-FITC and PI staining assays were preformed to quantitate the number of apoptotic cells.
Furthermore, we performed morphometric analysis to quantitate the number of neutrophils in the lung (Table 2).
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We used SBF-SEM to quantitate the numbers and mean lengths of collagen fibrils in embryonic (E15.5) WT and Mmp14 KO tendon, using methods described previously (Starborg et al., 2013).
To identify and quantitate the number of glomerular epithelial cells that express both a podocyte and PEC proteins (called glomerular epithelial transition cells), double immunostaining was performed as follows.
To determine which cells were responsible for reduced IL-17 production in mutant mice during bacterial pneumonia, we performed flow cytometry to quantitated the number and % of CD4+ T cells and γδ T cells expressing intracellular IL-17 in WT, TLR4lps-d, TLR9−/− and TLR4lps-d/TLR9−/− double mutant mice 24 hours post i.t.
When we do see profiles, we can quantitate the number of cells undergoing abnormal ciliogenesis (including profiles at stages 3/4/5) which is summarized as follows.
To this end we quantitated the number of BrdU+cells in the entire olfactory bulb (OB), the number of BrdU+/DCX+cells in the rostral migratory stream (RMS), the number of neuroblasts (immunostained for doublecortin, DCX) in the granular layer of the OB, and the number of mature interneurons (calretinin+cells) in the glomerular layer.
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