Sentence examples for to quantitate the expression of from inspiring English sources

Western blot was performed to quantitate the expression of retinal ICAM-1.

To this purpose, we used a low density DNA microarray specifically designed to quantitate the expression of genes involved in the inflammatory pathways and a customized real-time PCR array.

The present study was therefore designed to quantitate the expression of markers of microglia and astrocyte activation using real-time RT-PCR, immunoblot and immunohistochemical analysis in aging brain in response to brain injury.

Proteins were also isolated from the transformed colonies to quantitate the expression of interacting proteins by ELISA.

Real-time RT-PCR was used to quantitate the expression of six genes from 74 genes with differential expression in the lungs and livers of the three rodent species (Table S1).

To quantitate the expression of the miRNA primary transcripts and the miRNA mature forms, real-time RT-PCR was performed.

The semi-quantitative RT-PCR was firstly performed to quantitate the expression level of MTA1 mRNA in different segments of epididymis.

Quantitative RT-PCR was carried out to quantitate the expression level of selective ER-stress related genes: CHOP, PERK, ATF6, BiP, XBP1 and GADD34.

Real-time PCR was carried out to quantitate the expression levels of LacZ- CUG 400 and DMPK 11-15(CUG)300 transcripts.

To quantitate the expression level of mature miR-26b (MIMAthe00083), the isolated RNA was reverse transcribed and amplified by a two-step quantitative RT-PCR method using the Hairpin-it TM miRNAs qPCR Quantitation Kit (Genepharma, Shanghai, China) according to the manufacturer's protocol.

To further validate the microarrays results, we used PCR to quantitate the expression levels of 12 randomly selected EC-restricted genes in primary ECs and non-ECs.