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Hematoxylin & eosin-stained high-power fields was used to quantify cells.
Several studies employed FISH to quantify cells of AOB and NOB, and to determine cell-specific ammonium and nitrite oxidation rates (Altmann et al. [2003]; Daims et al. [2001]; Gieseke et al. [2005]; Wagner et al. [1995]).
To quantify cells which expressed Nanog, EBs were sectioned every 5 µm.
Tissue sections were viewed by light microscopy with an Olympus BX41 light microscope to quantify cells.
A crucial consideration is the difference in the methodology used by different groups to quantify cells.
Click-iT EdU Alexa Fluor 488 kit (Life Technologies) was used to quantify cells actively synthesizing DNA.
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At last, this approach allows us to quantify cell breathing and network densification.
Briefly, the assay is a non-radioactive, spectrophotometric method to quantify cell proliferation and viability in response to HU treatment.
LDH assay was used here in a modified version (after induced lysis of cells) to estimate number of viable cells to quantify cell growth (see Figure 5B).
Trypan blue exclusion was used to quantify cell membrane integrity after the addition and removal of CPA.
Broth samples (2 mL) were collected at 8-h intervals and were analyzed twice to quantify cell growth and lovastatin concentration.
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