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There are 5 members of the family, and although the community had already obtained mutations in 3 of the genes, we were able to provide mutations in the remaining 2, as well as additional alleles of the previously mutated 3. The nuclear hormone receptor (NHR) family with 275 genes is one of the larger gene families in the nematode.
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Large-scale projects to generate mutations in every C. elegans gene (reviewed in [ 10]) currently provide mutations in less than half of the genes.
When the Deletion Consortium was formed, its mission was to provide targeted mutations, which at the time was the only route to systematically examine loss-of-function phenotypes.
For example, the final release of mutations from the exome data will provide a perfect opportunity to provide comprehensive mutation class and pathogenicity score assignment across all TCGA samples.
To provide high-confident mutations, non-synonymous mutations identified in the WGS and WES sets were selected for TCS.
In this study, 'RetinoGenetics', which contains more genes and mutations, was developed to provide full-scale mutation information and extensive annotations for genetic retinal disorders.
For example, stronger selection on males is expected to improve the mean fitness of females for a given mutation rate, provided mutations have the same directional effect on fitness in each sex [ 15].
Our facilities provided mutations for 22 of these genes, and in 18 instances, we provided the canonical allele.
Although the specific length 100 is somewhat arbitrary, it is enough to provide space for mutations and significant adaptation.
Yeast knockouts were screened for genes that provide robustness to normal developmental perturbations [ 22]; these genes (sometimes known as 'phenotypic stabilizers' rather than capacitors because they have not yet been shown to provide robustness to mutations [ 23]) are enriched for a number of gene types, but not chaperones.
We used a semi-quantitative analysis that incorporated both the presence and clonality of all tested LOH mutations to provide an assessment of mutational load for a given microdissected target.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com