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Multiple ePBR units can be networked to perform parallel experiments, enabling semi-high throughput operations.
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The Flexostat implements eight parallel culture chambers, which enable users to gather replicate data and perform parallel experiments requiring no extra pumps or controllers.
The array allows to incorporate multiple negative controls in the course of a single binding experiment, as well as to perform parallel identical experiments to improve reliability of detection.
To ensure that these findings were not unique to A549 cells, we performed parallel experiments using the human bronchial epithelial cell line 16HBE to assess whether bronchial epithelial cells also undergo EMT during carbachol stimulation.
We performed parallel experiments to assess cell cycle perturbation when gemcitabine was combined with MK-8776.
To check the specificity of the CS2132 antibody, we performed parallel experiments on brain extracts from parkin-null mice.
We performed parallel experiments using purified GST tag to distinguish proteins selectively captured by RB1N, which identified 34 proteins that selectively interacted with RB1N (Table S1; Supplemental Experimental Procedures).
To ascertain the specificity of the N-AI effect, we performed parallel experiments by co-transfecting HCT116 cells with pGL3N2PR-2327/-99 and pCGN-HA-S33Y- β-catenin, a plasmid encoding an S33Y-mutated β-catenin protein known to activate the NOTCH2 promoter through the Wnt pathway.
However, to test the effects of PBA upon β-secretase processing directly, we performed parallel experiments examining AICD-GV16 induced Gal4-reporter activity and Aβ40 and Aβ42 production.
To examine the role of C5a-C5aR intheactiobservedhe observed inflammatory responses, we also performed parallel experiments including monoclonal antibody blockade of the C5a receptor.
As controls, we performed parallel experiments for EGFP and p53-GFP, a protein that transiently binds to DNA [42], [44].
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