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Per sample normalization was performed to normalize for staining intensity variations among samples.
The BMP-responsive luciferase output was normalized to renilla expression to normalize for transfection efficiency variation.
The copy number of MECP2 was normalized to that of FOXP2 to normalize for differences in DNA input.
We further note that it is possible to normalize for differences between sources using quantile normalization (Bolstad et al., 2003).
Ratios of pSmad to HA were calculated to normalize for variable transgene expression.
To normalize for differences in efficiency of sample extraction or cDNA synthesis we used β-actin as housekeeping gene.
The final results are presented relative to control samples to normalize for inter-assay variability.
The final results are presented relative to control to normalize for inter-assay variability.
GAPDH mAb (Proteintech Group , Inc was used to normalize for the amount of loaded protein.
The expression of ACTB was used as a reference to normalize for input cDNA.
Following transfection, luciferase activity was standardized to Renilla luciferase plasmid activity to normalize for transfection efficiency.
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