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To minimize the introduction of foreign components, we here develop a new CRISPR/dCas9-based system (CRISPR/dCas9-R2) to reduce the DNA methylation level by blocking the DNMT1 activity in the targeted loci (Fig. 1a).
In order to minimize the introduction of debris into the system, all the reservoir internals exposed to the oil should be noncorrosive.
To minimize the introduction of MS-incompatible salts, the samples were diluted 1000 1 with 18.2 MΩ water produced in-house.
Q5 high-fidelity DNA polymerase was used for all PCR reactions to minimize the introduction of possible error bases, and Sanger sequencing was applied for fidelity confirmation.
In order to make the SBR Tool work in the real-valued domain, additional tools are included that strive to minimize the introduction of aliasing in the SBR processing.
If variation in the species composition of communities (i.e., community context) is what often generates geographic variation, then we need to minimize the introduction of species, even species from nearby areas.
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To minimize the salt introduction which would dramatically reduce the solubility of the four buffer-insoluble SH3 mutants, most CD and NMR experiments were done at pH 4.0.
Despite the paucity of effects data from long-term, simultaneous exposure at low doses to multiple xenobiotics (particularly non-target-organism exposure to PPCPs), a wide range of proactive actions could be implemented to reduce or minimize the introduction of PPCPs to the environment.
This laboratory included a PCR set-up room that had established standard practices to minimize the potential for introduction of PCR product, plasmid and other possible PCR contaminants.
In the interim, marker data can assist with the selection of germplasm to minimize the risk of introductions and yet maintain high levels of genetic diversity.
The functions of the reservoir are to contain the required amount of system fluid in a manner that will minimize the introduction of debris into the system and to furnish turbulent free, degassed liquid to the pump suction piping at the specified conditions.
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